Influence of FfPpt1, FfSre1 and FfFtr3/FfFet3 on lysine biosynthesis, iron homeostasis and oxidative stress.

A: Growth ability of the indicated strains on solidified Czapek Dox (CD) medium supplemented as indicated. Representative pictures were taken after 3 days of incubation at 28°C in the dark. B: Phylograms of ferroxidases and iron permeases from F. fujikuroi (Ff), characterized proteins from F. graminearum (Fg) and A. fumigatus (Af), as well as homologous sequences from F. oxysporum (FOXG) and F. verticillioides (FVEG) obtained from the Broad Institute database were created as described in Methods. Scale bars represent character changes. C and D: Northern blot analysis using indicated genes as probes and rRNA visualization as loading control. The indicated strains were grown as described in Methods. (−); addition of water, (+) addition of FeCl3 to a final concentration of 1 mm.