Inflammatory property of Eutirucallin.

A. Eutirucallin (1–50 µg) was injected into the peritoneal cavity of the animals. Six hours later the total counts of harvested cells were performed by flow cytometry. The results are reported as the percentage of neutrophil per ml of cavity wash. PBS was used as controls. Adherent cells from the peritoneal cavities of C57BL/6 mice were stimulated in vitro for 48 h with Eutirucallin (1–50 µg/mL), medium (negative control) or LPS (positive control). The culture supernatants were assayed for NO (B), IL12p40 (C) and TNF-α (D) levels. *p<0.05,**p<0.01, and ***p<0.001 comparing with medium.