Infectivity and in vitro development illustration.

Representative photomicrographs of in vitro assay infection in Vero cell cultures with Berenice-62 and Berenice-78 parental strains and subpopulations/isolates of Be-78 strain, obtained from hemocultures at 3, 6 or 12 months after infection of Swiss mice with 5000 blood trypomastigotes from Be-78 Trypanosoma cruzi strain. After 18 hours exposure to the parasite, the cells were washed to remove extracellular parasites and maintained in medium until collection, fixed and stained at 24, 48 or 72 hours after inoculation. Increasing the rate of infectivity (asterisks) 48h after incubation for Be-62 strain (a, b, c) strain and for Be 78is5-3mai isolate (g, h, i); Increased rate of intracellular multiplication (asterisks) in 72h time for Be-78 parental strain (d, e, f); Infectivity and intracellular development profiles upper (asterisks) for isolate Be-78is2-6mai (j, k. G), and lower for Be-78is14-12mai (m, n, o). Fast Panotic. Bar = 25μm.