Induction of apoptotic molecules by deficiency of IFIT3.

A549 cells transfected with control siRNA (si-Ctl) or IFIT3 siRNA (siIFIT3) for 24 h were infected by mock or DV at M.O.I. = 5 for another 24 h. The cleaved proteins, including caspase 8, caspase 9, caspase 3 and BAX were determined by western blotting (A). Caspase 3 activity (B) or Annexin V and 7-AAD (C) were determined by flow cytometry analysis at postinfection 48 h. The representative results and the analysis pooled from at least three independent experiments were shown. The analysis was performed by ANOVA as described in Materials and Methods. *P<0.05, **P<0.01, ***P<0.001. Ctl stands for control.