Individual assembly of full-length env genes from a mixture of two variants.
(a) The length distribution of the synthetic long reads (minimum length 1 kb) shows assembly of full-length 3-kb env gene sequences. (b) 1,173 synthetic reads between 1.5 and 3.2 kb in length were aligned to each of the two original env sequences (env1 and env2). The alignment match rates are shown as a heatmap, with each synthetic read represented by a thin horizontal line. The majority of the synthetic reads align with low error to exactly one of the two original sequences, indicating high accuracy and a low rate of chimera formation. Chimeric reads would be expected to match both original sequences at intermediate accuracies. (c) Scatter plot showing the mismatch rates of each synthetic read against the two known env sequences. Synthetic reads (open circles to emphasize extensive overlap) cluster into two distinct groups along the axes (near-zero mismatch rate). Even the sixteen reads that do not fall on the clusters are distant from three manually created mock chimeras (crosses), indicating a low frequency of chimera formation.