Increased kyn release of SKOV-3 cells upon 1-D-MT treatment.

(A) Kyn concentrations released by SKOV-3 cells after treatment with 1-D-MT (white circles), 1-L-MT (black circles) and the racemic mixture of 1-MT (black triangles) measured after 48 h by HPLC. (B) Kyn release of SKOV-3 cells in response to 500 µM 1-D-MT in the presence of increasing trp concentrations. (C) Kyn release of SKOV-3 cells treated with different concentrations of trp alone (open circles) or in combination with 1 mM 1-D-MT (filled circles) measured after 48 h by HPLC. (D) Kyn production in IDO1 enzymatic assays performed in the presence of 100 µM trp in combination with increasing 1-D-MT concentrations. (E) Kyn production of IDO1 enzyme in the presence of increasing concentrations of trp alone (open circles) or in combination with 1-D-MT (filled circles). Experiments were performed in triplicate. Data are mean ± SEM. * (p<0.05).