Increased CTGF and collagen staining decreases after anti-inflammatory drug treatments.

<p>CTGF immunohistochemistry (panels A–F) and Masson's trichrome staining (panels G,H) increased in flexor digitorum muscles (M) and lumbricals in TR-HF and 6-week HRHF task rats, and decrease with anti-inflammatory drugs. Panels A–F show CTGF immunostaining (red) of flexor digitorum muscles from: <i>(A)</i> TR-HF, <i>(B)</i> TR-HF treated with anti-TNF-α, <i>(C)</i> untreated 6-week HRHF (showing CTGF-immunostained cells at edges of myofibers), <i>(D)</i> 6-week HRHF treated with anti-TNF-α (inset shows small CTGF-immunostained cells in endomyseum), <i>(E)</i> 6-week HRHF treated with ibuprofen, and <i>(F)</i> untreated 6-week HRHF (showing increased CTGF-immunostained cells in endomyseum (ct)). <i>(G)</i> Quantification of percent area with CTGF immunostaining. **p<0.01 compared to NC; <sup>##</sup> p<0.01 compared to TR-HF; <sup>&&</sup>p<0.01 compared to untreated 6-week HRHF; n = 4–12/gp. <i>(F)</i> Lumbricals from Region B stained with Masson's Trichrome showing: <i>(H)</i> increased blue stained collagen matrix in an untreated 6-week HRHF rat, and <i>(I)</i> decreased blue staining in a 6-week HRHF treated with anti-TNF-α. Scale bars = 50 µm.</p>