In vivo administration of GB induces spleen DC activation.

C57BL/6 mice were injected i.p. with 10 or 50 mg/kg GB for 24 hours. (A) Percentage of lineage^-CD11c⁺ spleen DCs was analyzed on a flow cytometry (left panels). Absolute cell number of lineage^-CD11c⁺ cells within live cells were shown (right panel). (B) Expression levels of CD40, CD80, CD86, MHC class I and MHC class II were measured by flow cytometry. Data are representative of analyses of 8 independent samples. (C) C57BL/6 mice were treated orally, i.p. or i.v. with 50 mg/kg GB for 24 hours and measured Expression levels of CD40, CD80, CD86, MHC class I and MHC class II by flow cytometry. (D) Expression levels of IL-6, IL-12p40 and TNF-α mRNA were measured from spleen 2 hours after 50 mg/kg GB injection. (E) IL-6, IL-12p70 and TNF-α levels in sera were shown from spleen 24 hours after 50 mg/kg GB injection. (F) Intracellular cytokine production levels were measured spleen DCs. All data are from analyses of 6 individual mice each group (2 mice per experiment, total 3 independent experiments). *p < 0.05, **p < 0.01.