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In vivo activation of NCL probe by luciferase and nitroredictase expressing E.coli in a mouse model of thigh infection.

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posted on 2015-06-25, 03:40 authored by Anzhelika G. Vorobyeva, Michael Stanton, Aurélien Godinat, Kjetil B. Lund, Grigory G. Karateev, Kevin P. Francis, Elizabeth Allen, Juri G. Gelovani, Emmet McCormack, Mark Tangney, Elena A. Dubikovskaya

(A). Luminescence over 4 h from E. coli luc+ infected quadriceps (5 × 104–5 × 107 bacteria) after IP injection of 0.8 mg NCL probe (200 μL of 10 mM solution in PBS). (B). Luminescence over 4 h from E. coli luc+ infected quadriceps (5 × 107 bacteria) following IP injection of 0.8 mg of probe or 0.63 mg of luciferin (200 μL of 10 mM solution in PBS). (C). Luminescence imaging of mice over 24 h bearing 5 × 107 bacteria, treated with various (0.08, 0.8 and 1.6 mg) NCL probe concentrations (200 μL of 1, 10 and 20 mM solutions of NCL in PBS). As a positive control, mice were injected with equal amounts of E. coli MG1655 expressing lux luciferase that doesn't require exogenous substrate for light production [44]. The signal was collected over 24 h, n = 3 per group.

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