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In vitro cultures of palatal explants from Bmp7 show more Δ/Δ embryos.

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posted on 2013-03-15, 09:20 authored by Thaleia Kouskoura, Anastasiia Kozlova, Maria Alexiou, Susanne Blumer, Vasiliki Zouvelou, Christos Katsaros, Matthias Chiquet, Thimios A. Mitsiadis, Daniel Graf

(A) Pairs of palatal shelves dissected from developmental stages E14.5 to E16.5 were placed in apposition in vitro and cultured. The results showed that Bmp7Δ/Δ palatal shelves retain the ability to fuse even at E16.5. (B) An example of a Bmp7Δ/Δ palatal shelf from E14.5 is shown at the start (0 h), after 20 h and 84 h of culture. Fusion at the midline is visually evident at 84 h and the formation of palatal rugae indicates continuing palatal development. Scale bar: 500 μm (C) Explants were sectioned coronally and stained for Keratin14. Continuity of the epithelial layer and disappearance of the medial epithelial seam indicate successful fusion.

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