Immunostaining for TPP1.

<p><i>Tpp1</i>(−/−) mice were administered unmodified enzyme or vehicle alone via tail vein injection and tissues collected after 24 hours. Tissue processing and immunostaining was conducted as described previously <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040509#pone.0040509-Sleat3" target="_blank">[33]</a>. Top Panels show the choroid plexus and hippocampus (original magnification 4×) with inset showing hippocampal CA2 neurons (20×). Bottom Panels show cerebral cortex layer 2/3 (20× magnification) with inset showing select neurons (60×).</p>