Immunolocalization of NTPD2 in different regions of the bladder.

Cryosections of mouse bladders were labeled with antibodies to NTPD2 (green), rhodamine phalloidin to label the actin cytoskeleton (red) and Topro-3 to label nuclei (blue). Color merged panels are shown on the right. A) NTPD2 staining at the level of the urothelium, B) NTPD2 staining at the level of the lamina propria; white asterisks (*) indicate areas positive for actin but negative for NTPD2 in the lamina propria. C) NTPD2 staining at the level of the detrusor smooth muscle. White scale bars  = 10 µm.