Immunohistochemistry of NeuN (A, B) and GFAP (C through F) in the OB.

Distribution of NeuN-positive neuronal cells do not reveal any obvious differences between NPC1+/+ (A) and NPC1−/− mice (B) (67–70 d). There is a clear increase of GFAP expression, especially in the glomerular layer (Gl) in NPC1−/− animals (D) compared to NPC1+/+ (C). The area marked by the rectangles is enlarged in E and F, respectively, demonstrating the astrogliosis of the granular layer (Gr) in NPC1−/− mice. Counterstained with hematoxylin.