Immunohistochemical staining of nerve grafts.

(A) Immunohistochemical staining of collected nerve grafts from sham control and xenotransplanted mice, were immunostained using anti-IFN-γ, anti-IL-17, and anti-IL-4 antibodies with DAB as the chromogen with a Hematoxylin counterstain. Both antibodies demonstrated scattered immunoreactivity in the infiltrating mononuclear cells. Representative images are shown. Magnification: 200×. (B) IFN-γ+ cells and IL-17+ cells increased in xenotransplanted group from day 6 to day 30 compared to the sham controls. There is no significant difference of IL-4+ cells between the recipients and control group. *, p<0.05; **, p<0.01; *** p<0.001. (We performed experiment twice, and stained 2 sections for IFN-γ and IL-17 respectively of each animal.)