Immunofluorescent localization of tubulin and KIFC1 in mature <i>E. modestus</i> sperm.

2013-09-30T03:03:24Z (GMT) by Cong-Cong Hou Wan-Xi Yang
<p>(A) DAPI nuclear staining. (B) Tubulin staining. Tubulin (green staining; arrows) localizes specifically to the center of the acrosome. (C) KIFC1 staining. KIFC1 (red staining; arrows) localizes throughout the whole acrosome including the AFS-derived spike (D) Phase contrast microscopy. This figure shows the general morphological appearance of the <i>E. modestus</i> mature sperm. (E) Merged immunofluorescent images. Tubulin (green staining; arrows) and KIFC1 (red staining; arrows) localization is shown. Nucleus (blue staining). (F) Diagram. This diagram shows the distribution of tubulin (green) and KIFC1 (red dots) in the <i>E. modestus</i> mature sperm. N: nucleus, MF: microfilaments AFS: acroframosome, C: centriole.</p>