Fig_7.tif (5.59 MB)
Immunofluorescence of human primary myoblasts in culture with GM and DM with or without anti-Syncytin-1 (-/+ Ab) for 4 days and analysis by confocal microscopy for nuclei (Hoechst 33342), F-actin (Phalloidin Alexa488), membrane (wheat germ agglutinin Alexa594) and the merge with all.
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posted on 2015-07-08, 02:50 authored by Sebastian Frese, Matthias Ruebner, Frank Suhr, Thierry M. Konou, Kim A. Tappe, Marco Toigo, Hans H. Jung, Christine Henke, Ruth Steigleder, Pamela L. Strissel, Hanna Huebner, Matthias W. Beckmann, Piet van der Keylen, Benedikt Schoser, Thorsten Schiffer, Laura Frese, Wilhelm Bloch, Reiner StrickNote the multinucleated myofibre in DM. DM composite of 96 z-stacks and GM of 17 z-stacks. Bars = 50μm.
