Figure_3.tif (1.79 MB)
Immunoblotting of LC-3 protein in INS-1E cells after 2, 6 and 12 h treatment with various concentrations of palmitate (PA).
figure
posted on 2013-02-20, 05:43 authored by Luisa Martino, Matilde Masini, Michela Novelli, Pascale Beffy, Marco Bugliani, Lorella Marselli, Pellegrino Masiello, Piero Marchetti, Vincenzo De Tataα-tubulin was used as a control for loading. Methodological details are described in Materials and Methods. The figure is representative of gels obtained from three separate experiments. From these gels, band intensities were evaluated by densitometric analysis and expressed as the mean ± SEM of the ratio between LC3-II and LC3-I bands (lower graphs). * p<0.01 vs. untreated control; § p<0.01 vs. PA 0.5 mM.