Immunoblot analyses of the wild type PrtV protein and its PKD domain deletion mutant in culture supernatants and OMV samples.

<p>Immunoblot analysis was performed using anti-PrtV antiserum (A) and anti-OmpU antiserum (B) with the following samples: lanes 1–3: <i>V</i>. <i>cholerae</i> Δ<i>prtV</i> strain carrying the cloned wild type allele of <i>prtV</i>; lanes 4–6: the Δ<i>prtV</i> strain carrying the cloned <i>prtV</i>ΔPKD allele; lanes 7–9: the Δ<i>prtV</i> strain carrying the pBAD18 cloning vector. Lanes 1, 4 and 7 were loaded with supernatant samples before ultracentrifugation (Sup1). Lanes 2, 5 and 8 were loaded with the supernatants after ultracentrifugation (Sup2). Lanes 3, 6 and 9 were loaded with the OMV samples.</p>