Immunization of Mice with TMV-Multiconjugate Vaccine Induces Antibody Responses Capable of Recognizing both Native and Recombinant OmpA, DnaK and Tul4 Proteins.

<p><b>(A)</b> Serum collected on day 28 post-immunization from C57BL/6 mice immunized with TMV-multiconjugate vaccine (Schedule II) was pooled (n = 4) and blotted against <i>F</i>. <i>tularensis</i> LVS and SchuS4 lysates. <b>(B)</b> Pooled serum from C57BL/6 mice (n = 4) immunized either with TMV-multiconjugate vaccine, or 100 CFU of <i>F</i>. <i>tularensis</i> LVS were collected on day 28 post immunization and blotted against purified recombinant OmpA, DnaK and Tul4 proteins. Sera from mice inoculated with TMV alone were used as controls.</p>