Immunization of Mice with TMV-Multiconjugate Vaccine Induces Antibody Responses Capable of Recognizing both Native and Recombinant OmpA, DnaK and Tul4 Proteins.

(A) Serum collected on day 28 post-immunization from C57BL/6 mice immunized with TMV-multiconjugate vaccine (Schedule II) was pooled (n = 4) and blotted against F. tularensis LVS and SchuS4 lysates. (B) Pooled serum from C57BL/6 mice (n = 4) immunized either with TMV-multiconjugate vaccine, or 100 CFU of F. tularensis LVS were collected on day 28 post immunization and blotted against purified recombinant OmpA, DnaK and Tul4 proteins. Sera from mice inoculated with TMV alone were used as controls.