Ih gene is expressed in dopaminergic neurons.

(A) Confocal projection of adult brain showing dopaminergic neurons labeled with GFP using the Tyrosine Hydroxilase Gal4 driver (THG4;UAS:GFP). D, dorsal, L, lateral, OE, oesophagus, OL, optic lobe. (B) RT-PCR amplification of Ih (112 bp) and ple (133 bp) RNAs from 200 isolated dopaminergic and non-dopaminergic neurons sorted by Fluorescence Activated Cell Sorting (FACS). Image shows amplification of Ih RNA in dopaminergic, ple-expressing neurons. Failure to PCR-amplify the neuronal ple RNA isoform in non-dopaminergic neurons (GFP negative cells) confirms that cells have been correctly sorted. Asterisks in lanes 1, 2, and 5 point to nonspecific bands, which were obtained in the negative controls (DmIh mutant brain-RNA extract for Ih –lane 1- and GFP-negative cell-RNA extract for ple –lane 5-).