Identification of nanoATV within spleen and lymph node.

Confocal microscopy images of cryosectioned spleen and mesenteric lymph nodes collected 12 h after intraperitoneal injection of CF633-labeled nanoATV (pink). Cell nuclei were stained with DAPI (blue); macrophages were stained with Alexa Fluor 488-linked F4/80 antibody (green). Immunostainings as follows: (A) H&E of spleen; (B) F4/80 labeled splenic macrophages; (C) CF633-nanoATV within spleen; (D) Overlay of (B) and (C); (E) F4/80 labeled splenic macrophages; (F) CF633-nanoATV in spleen; (G) overlay of (E) and (F); (H) H&E of lymph nodes; (I) overlay of F4/80 labeled macrophages and CF633-nanoATV in lymph nodes; (J) CF633-nanoATV within lymph nodes; (K) overlay of F4/80 labeled macrophages and CF633-nanoATV in lymph nodes. Abbreviations: RP (red pulp), MZ (marginal zone), F (B-cell rich), T (T-cell rich), M (medulla), and SCS (subcapsular sinus). Magnifications: 40X for B, C, D, J and K; 10X for A, E, F, G, H and I. (scale bars = 100 μm).