Fig_5.tif (4.83 MB)

Identification of nanoATV within spleen and lymph node.

figure

posted on 2016-01-05, 14:53 authored by Andrea L. Martinez-Skinner, Mariluz A. Araínga, Pavan Puligujja, Diana L. Palandri, Hannah M. Baldridge, Benson J. Edagwa, JoEllyn M. McMillan, R. Lee Mosley, Howard E. Gendelman

Confocal microscopy images of cryosectioned spleen and mesenteric lymph nodes collected 12 h after intraperitoneal injection of CF633-labeled nanoATV (pink). Cell nuclei were stained with DAPI (blue); macrophages were stained with Alexa Fluor 488-linked F4/80 antibody (green). Immunostainings as follows: (A) H&E of spleen; (B) F4/80 labeled splenic macrophages; (C) CF633-nanoATV within spleen; (D) Overlay of (B) and (C); (E) F4/80 labeled splenic macrophages; (F) CF633-nanoATV in spleen; (G) overlay of (E) and (F); (H) H&E of lymph nodes; (I) overlay of F4/80 labeled macrophages and CF633-nanoATV in lymph nodes; (J) CF633-nanoATV within lymph nodes; (K) overlay of F4/80 labeled macrophages and CF633-nanoATV in lymph nodes. Abbreviations: RP (red pulp), MZ (marginal zone), F (B-cell rich), T (T-cell rich), M (medulla), and SCS (subcapsular sinus). Magnifications: 40X for B, C, D, J and K; 10X for A, E, F, G, H and I. (scale bars = 100 μm).