Identification of nanoATV within spleen and lymph node.

<p>Confocal microscopy images of cryosectioned spleen and mesenteric lymph nodes collected 12 h after intraperitoneal injection of CF633-labeled nanoATV (pink). Cell nuclei were stained with DAPI (blue); macrophages were stained with Alexa Fluor 488-linked F4/80 antibody (green). Immunostainings as follows: <b>(A)</b> H&E of spleen; <b>(B)</b> F4/80 labeled splenic macrophages; <b>(C)</b> CF633-nanoATV within spleen; <b>(D)</b> Overlay of (B) and (C); <b>(E)</b> F4/80 labeled splenic macrophages; <b>(F)</b> CF633-nanoATV in spleen; <b>(G)</b> overlay of (E) and (F); <b>(H)</b> H&E of lymph nodes; <b>(I)</b> overlay of F4/80 labeled macrophages and CF633-nanoATV in lymph nodes; <b>(J)</b> CF633-nanoATV within lymph nodes; <b>(K)</b> overlay of F4/80 labeled macrophages and CF633-nanoATV in lymph nodes. Abbreviations: RP (red pulp), MZ (marginal zone), F (B-cell rich), T (T-cell rich), M (medulla), and SCS (subcapsular sinus). Magnifications: 40X for <b>B, C, D, J</b> and <b>K</b>; 10X for <b>A, E, F, G, H and I.</b> (scale bars = 100 μm).</p>