Identification of nanoATV in cells after intramuscular administration.

<p>Balb/c mice injected intramuscularly with 100 mg/kg CF633-nanoATV were sacrificed on days 1 and 7 after injection. Cryosections were prepared for H&E staining and for confocal microscopy. <b>(A)</b> Flow cytometric analysis of spleen demonstrates significant uptake of nanoATV in macrophages (CD45+CD3-CD11b+F4/80+) compared to lymphocytes (CD45+CD3+) and neutrophils/granulocytes (CD45+CD3-CD11b+F4/80-) at days 1 and 7. Data are represented as mean per mouse ± SEM and considered significant at P<0.05 (Student’s t-test). <b>(B)</b> Cryosections of muscle at the site of injection stained with H&E; 40X. <b>(C)</b> The muscle cryosection was stained with DAPI for nuclei (blue). The red arrowheads show the accumulation of nanoATV at the site of injection, 63X. <b>(D)</b> Macrophages were immunostained with CD68 antibody and Alexa Fluor 488 secondary antibody. Colocalization of nanoATV (pink) and CD68+ cells (green) at the site of injection is indicated with red arrowheads. Nuclei were stained with DAPI (blue), 63X. (scale bars = 10 μm)</p>