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IL-2 complexes relocate B cells from the bone marrow to secondary lymphoid organs.

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posted on 2016-01-18, 14:26 authored by Benedikt Mahr, Lukas Unger, Karin Hock, Nina Pilat, Ulrike Baranyi, Christoph Schwarz, Svenja Maschke, Andreas Michael Farkas, Thomas Wekerle

The mechanisms of B cell expansion in secondary lymphoid organs were investigated. Naive C57BL/6 mice received IL-2 complexes (5μg IL-2 / 25μg α-IL-2) for three days (d0, d1, d2) and distinct cell populations were analyzed in indicated tissues 2 days (d4) after the last dose. (A) CD3+ CD4+ Foxp3 T cells, CD3+ CD8+ T cells, CD3 Nk1.1+ NK cells and CD3+ NK1.1+ cells but not CD19+ B cells proliferated upon IL-2 complex treatment in the spleen as assessed by their expression of Ki67. Histograms are shown from representative mice (n = 4). (B) IL-2 complexes (n = 4) reduced the proportion and absolute number of CD19+ B cells within CD45+ leukocytes in the BM as compared with untreated mice (n = 4). Two color dot plots illustrate representative mice (left). Data are pooled from 2 independent experiments (right) [p = 0.014].

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