IL-27-induced mRNA expression of gp130 is impaired in HIV infection.

(A) Purified blood monocytes were stimulated with IL-27 for 4, 8, and 16 h, then analyzed by real-time PCR for mRNA expression of gp130 (n = 4). (B) Monocytes from HIV negative (n = 8) and HIV positive subjects (n = 7) were stimulated for 4 h with IL-27, and then analyzed by real-time PCR for gp130 mRNA expression. Comparison of gp130 expression between the two groups was performed with an independent samples t-test. Data shown have been normalized to 18SrRNA expression, and represent IL-27-mediated induction of gp130 expression relative to expression levels in unstimulated cells (Medium).