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IL-12, IL-4 and IL-10 responses in BALB/c mice vaccinated with different vaccine regimens before and after 3 months challenge infection.

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posted on 2013-02-20, 20:51 authored by Saumyabrata Mazumder, Mithun Maji, Amrita Das, Nahid Ali

Ten days, short-term protection (STP), and twelve weeks, long-term protection (LTP) after final boosting (post-vaccination), and 3 months after challenge infection (post-infection) splenocytes were collected from vaccinated mice, stimulated with rgp63 (5 µg/ml) and were cultured for 96 h. The supernatants were collected, and assayed for IL-12 (A–D), IL-4 (E–H), and IL-10 (I–L) through ELISA. The results are shown as the mean absorbance values ± S.E. of five individual mice per group, representative of two independent experiments with similar results. OV- only vector. * p<0.05, ** p<0.01, *** p<0.001 as assessed by one-way ANOVA and Tukey's multiple comparison test.

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