IFN-γ and NO production exhibit patterns mostly similar to that of in vitro LVS replication.

Supernatants from co-cultures described in Fig 1 using splenocytes of BALB/cByJ mice (Panels A and C) and BKO mice (Panels B and D) were collected after two days of co-culture, and separated from cells for analyses of IFN-γ by ELISA (Panels A and B) and NO by Griess reaction (Panels C and D). Concentrations were calculated using standard curves as reference. Values shown are the mean concentration in ng/ml (IFN-γ) or μmoles (NO) ± standard deviation of triplicate samples. Results shown are from one representative experiment of seven (using splenocytes of BALB/cByJ mice) or four (using splenocytes of BKO mice) independent experiments of similar design and outcome. Brackets indicate a significant difference (P < 0.05) between amounts of IFN-γ or NO produced in co-cultures. There were no significant differences in IFN-γ production between the co-cultures using LVS-immune cells and the co-cultures using LVS-G-immune cells (Panel A), between the co-cultures using LVS-G-immune cells and the co-cultures using LVS-R-immune cells (Panel A), and between the co-cultures using LVS-immune cells and the co-cultures using LVS-G-immune cells or LVS-R-immune cells (Panel B).