IFN-γ and IL-17A ELISPOT cells in spleen and draining lymph nodes of mice vaccinated with MGM1991 or BCG.

IFN-γ and IL-17A producing cells in spleen and inguinal lymph nodes of C57BL/6 mice vaccinated 4 weeks before with 5x104 CFU of MGM1991 or M. bovis BCG by the subcutaneous route or from unvaccinated (naïve) mice and stimulated in vitro with recombinant Ag85A (5 µg/ml), with I-Ab restricted immunodominant peptide spanning aa 1-20 of ESAT-6 or with purified protein derivative PPD from M.tb (10 µg/ml). Cytokine levels are expressed as mean number of spot forming cells/ 106 spleen cells (5 mice tested individually) or as mean number of spot forming cells/ 5x105 lymph node cells (pooled cells from 5 animals/group). * p < 0.05 (Mann-Whitney test).