IFN-γ and IL-17A ELISPOT cells in spleen and draining lymph nodes of mice vaccinated with MGM1991 or BCG.
2013-10-17T04:37:21Z (GMT) by
<p>IFN-γ and IL-17A producing cells in spleen and inguinal lymph nodes of C57BL/6 mice vaccinated 4 weeks before with 5x10<sup>4</sup> CFU of MGM1991 or <i>M. bovis</i> BCG by the subcutaneous route or from unvaccinated (naïve) mice and stimulated in vitro with recombinant Ag85A (5 µg/ml), with I-A<sup>b</sup> restricted immunodominant peptide spanning aa 1-20 of ESAT-6 or with purified protein derivative PPD from <i>M.tb</i> (10 µg/ml). Cytokine levels are expressed as mean number of spot forming cells/ 10<sup>6</sup> spleen cells (5 mice tested individually) or as mean number of spot forming cells/ 5x10<sup>5</sup> lymph node cells (pooled cells from 5 animals/group). * <i>p</i> < 0.05 (Mann-Whitney test).</p>