ICP0 co-localizes with reorganized microtubules in HSV-1 infected cells.

<p>Immunofluorescent staining of Vero cells that were uninfected (no virus) or that were inoculated with 5 pfu per cell of HSV-1 0ΔRING, HSV-1 0<sup>+</sup>GFP<sub>105,</sub> or wild-type HSV-1 strain KOS. Cells were inoculated in the presence of 200 µM cycloheximide from −0.5 to 10 hours p.i., and were released into medium containing no drugs. At 4 hours post-release, uninfected cells and KOS-infected cells were fixed and stained with antibodies against α-tubulin (rabbit IgG, Alexa Fluor 594) and ICP0 (mouse IgG, fluorescein). Nuclei were counterstained with the DNA-binding dye Hoechst 33342. HSV-1 0ΔRING and 0<sup>+</sup>GFP<sub>105</sub>-infected cells were fixed and stained for α-tubulin, and the ICP0<sup>ΔRING</sup> and ICP0<sup>+GFP-105</sup> proteins were visualized using their GFP fluorophores. The scale bar denotes a distance of 10 µm.</p>