Hypoxia/reoxygenation (H/RO) induced apoptotic death in NARP cybrids and 143B cells.

(A) H/RO (H: 6h, RO: 2h) induced apoptosis in NARP cybrids as measured by Annexin V-propidium iodide (PI) staining. (B) H/RO induced cytochrome c release from mitochondria in NARP cybrids and 143B cells as measured by immunocytochemical analysis. Red color: mitochondrial complex II; Green color: cytochrome c. Note that cytochdrome c and complex II co-localized well before H/RO (yellow color). After H/RO, green fluorescent signal was released from mitochondria in NARP cybrids and 143B cells, which suggested that cytochdrome c was released from mitochondria. (C) MTT assay. After H/RO, cell death was noted in NARP cybrids and 143B cells (death ratio: NARP > 143B). (D) Trypan blue exclusion test of cell viability. After H/RO, cell death was noted in NARP cybrids and 143B cells (death ratio: NARP > 143B). *P<0.05 as compared with control; n=3.