Human Fibroblasts and Fibrosarcoma Cells bound to Fibronectin also Align in Defined Cation Conditions.

(A) Cell density of human fibroblasts (WI38) and human fibrosarcoma cells (HT1080) attached to fibronectin were plotted as a function of the applied shear stress. Cation conditions are indicated. Note that each representative curve represents thousands of cells grouped together at set radial distances with data expressed as mean ± standard deviation. (B) For the indicated cation and shear stress conditions, fluorescent images of 3T3 fibroblasts, WI38 fibroblasts, and HT1080 fibrosarcoma cells are shown. Cells were stained for DNA (blue) and actin (red). The direction of shear is indicated for cells subjected to higher stress (center and right images). Some aligned cells are indicated by open arrowheads. Dashed lines indicate the regions of inset images, which are outlined in white.