Hit selection and therapeutic class of drugs from primary HTS assay for ISRE activation.

(A) Plot of z-scores for each of the 2240 compounds subjected to the primary screen for ISRE activation. Values represent mean of two replicates per compound concentration. Compounds are ranked by highest z-score achieved at any of the four compound concentrations tested. Dashed lines demarcate the compounds with z-scores greater than 2 SD above the mean; and red color denotes the 64 hit compounds selected from this group for validation. (B) The distribution of the 64 hit compounds into various therapeutic classes. Values indicate the number of compounds in each class.