Figure_3.tif (1.72 MB)

High dimensional analysis of single cells from breast cancer cell lines.

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posted on 2012-05-07, 00:35 authored by Ashley A. Powell, AmirAli H. Talasaz, Haiyu Zhang, Marc A. Coram, Anupama Reddy, Glenn Deng, Melinda L. Telli, Ranjana H. Advani, Robert W. Carlson, Joseph A. Mollick, Shruti Sheth, Allison W. Kurian, James M. Ford, Frank E. Stockdale, Stephen R. Quake, R. Fabian Pease, Michael N. Mindrinos, Gyan Bhanot, Shanaz H. Dairkee, Ronald W. Davis, Stefanie S. Jeffrey

A. Heatmap of single cell gene expression of 87 genes within seven individual cells isolated from three primary tumor-derived (pink: CCdl054, orange: CCdl672, gold: CCdl675), and four metastatic effusion-derived (red: MDA-231 plum: SKBR3, dark green: MCF7, and bright green: T47D) breast cancer cell lines. Yellow indicates high gene expression; gray is median expression; blue indicates low expression; and black represents undetectable expression. All cells showed expected expression patterns. The breast cancer cell lines used represent a spectrum of cell differentiation, e.g., from less differentiated and more mesenchymal/stem cell-like ER-negative (basal-like) cells (MDA-231 and SKBR3) to more differentiated ER-positive (luminal-like) cells represented by CCdl054, CCdl672, CCdl675, MCF7, and T47D.