Heparin effects during oligodendrogenesis and oligodendrocyte maturation in CNP::EGFP mice cultures.
A) The number of NG2+ cells at the end of the proliferation stage are expressed as a proportion respect to the total number of nuclei analyzed per treatment. More than 300 nuclei were analyzed for each treatment in 4 independent cultures. B, C) The proportion of GFP+ and MBP+ cells after differentiation is compared in Heparin (1.25 U/L) treated cultures against culture lacking Heparin. More than 600 nuclei were analyzed for a each treatment. Data analysis in graphs was performed using Student´s t Test. D) Representative images of CNP::EGFP mice derived cutlures demonstrating EGFP+ (green) and MBP+ (magenta) cells. Scale bar in D (i) represents 100 μm for all images in D. Each pair of columns in A (with or without Heparin) was analyzed with a Student´s t test. ns = not significant.