H&E staining and osteocalcin immunolocalization on explants retrieved 7 and 21 days after transplantation of cell sheet-based constructs.

(A–D) H&E staining on (A;B )control and (C;D) experimental explants after 7 (A;C) and 21 days (B;D) of subcutaneous implantation showing their localization and morphology. (E–L) Immunolocalization of osteocalcin on (E;F) control and (G;H) experimental explants at 7 (E;G) and 21 (F;H) days of implantation revealing osteogenic commitment on both test conditions. (I–L) immunostaining negative control of respective E–H conditions.