HPLC/MS-MS Analysis of Dominant Follicle Sex Steroid Concentrations Collected after Ovariectomy.

Differences in concentration of androgens (A) and estrogens (B) was determined by HPLC/MS-MS (Biocrates Life Sciences AG) analysis for High A4 (black bars) and Low (white bars) A4 cows. *, P≤0.05.