HDAC inhibition induces cell cycle arrest and reduces colony forming ability in AML cells.

THP-1 cells were treated with chidamide at 1 µM or VPA at 1 mM for 24 h or 48 h. A. Cell cycle of THP-1 cells was determined by FACS analysis based on DNA content. Cell cycle was presented as percentage of cells in G1/S/G2&M phase. B. Western blot analysis on CDK2 and CDK4. C, D. Apoptosis of THP-1 cells after chidamide or VPA treatment. E, F. Colony forming analysis of THP-1 cells. Number of clone (≥50 cells) per well (E) as well as representative photographs in each group (F) were shown. *P<0.05, **P<0.01.