Growth assays of S. cerevisiae strains expressing V. vinifera aquaporins on H2O2 containing minimal media.
Yeast strain transformed with empty pUG35 plasmid was used as control (pUG35). Yeast suspensions were spotted in 10-fold dilution on solid YNB plates without or with 0.75 mM and 1.0 mM H2O2. Growth was recorded after two weeks at 28°C. Photographs shown are representative of three independent experiments with consistent results.