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Growth assays of S. cerevisiae strains expressing V. vinifera aquaporins on H2O2 containing minimal media.

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posted on 2014-08-11, 03:38 authored by Farzana Sabir, Maria José Leandro, Ana Paula Martins, Maria C. Loureiro-Dias, Teresa F. Moura, Graça Soveral, Catarina Prista

Yeast strain transformed with empty pUG35 plasmid was used as control (pUG35). Yeast suspensions were spotted in 10-fold dilution on solid YNB plates without or with 0.75 mM and 1.0 mM H2O2. Growth was recorded after two weeks at 28°C. Photographs shown are representative of three independent experiments with consistent results.

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