Growth assays of S. cerevisiae strains expressing V. Vinifera aquaporins on minimal medium containing boric acid.

Yeast strain transformed with empty pUG35 plasmid was the control (pUG35). Yeast suspensions were spotted in 10-fold dilution on solid YNB plates without or with 40 mM and 60 mM boric acid. Growth was recorded after two weeks at 28°C. Photographs shown are representative of three independent experiments with consistent results.