Granule cell precursor proliferation was decreased in the cerebellums of <i>Rere</i><sup>om/eyes3</sup> embryos at E18.5.
2014-01-23T04:44:19Z (GMT) by
<p>A-F. Proliferation assays were performed on cerebellar vermis sections prepared from the cerebellums of wild-type and <i>Rere</i><sup>om/eyes3</sup> embryos and mice at E18.5 (A–B), P0 (C–D) and P3 (E–F) using an anti-Phospho Histone H3 antibody. A-B. The number of mitotic granule cell precursors in <i>Rere</i><sup>om/eyes3</sup> embryos appeared to be lower than that of their wild-type littermates at E18.5. C-F. Mitotic activity of GCPs was comparable between <i>Rere</i><sup>om/eyes3</sup> embryo and wild-type littermate at P0 and P3. G. Phospho-Histone H3-positive cells in the entire external granule cell layer (EGL) were counted and normalized by the area of the EGL (n≥3 with twenty slides containing three sections for each genotype). The number of Phospho-Histone H3-positive GCPs per µm<sup>2</sup> was reduced in <i>Rere</i><sup>om/eyes3</sup> embryos at E18.5 when compared to wild-type littermates (* = <i>p</i><0.03) but was indistinguishable between <i>Rere</i><sup>om/eyes3</sup> embryos and wild-type littermates at P0 and P3. ABL, anterobasal lobe; ADL, anterodorsal lobe. Scale bar = 200 µm.</p>