Germline chimeras and offspring from PαS-iPS cells.
2011-03-11T00:26:43Z (GMT) by
<p><b>A</b>, Chimeric and F1 mice from 4F-PαS-iPS and 3F-PαS-iPS cells. 3F-PαS-iPS could observe nine over 50% chimerism chimeras. <b>B</b>, Germline-competent chimeric mice and F1 mice. Seven chimeras (>50% coloured coat colour) from 3F-PαS-iPS #1 and two from 3F-PαS-iPS #2 were bred with ICR females, and three of seven chimeas from 3F-PαS-iPS #1 and one of two chimera from 3F-PαS-iPS #2 produced iPS derived F1 mice. The ratio of pigmented F1 mice was observed. Numbers below each panel give the percentage of pigmented F1 mice. <b>C</b>, PCR analysis showing the presence of the GFP cassette in F1 mice obtained from the intercross between a chimeric male and an ICR female. <b>D</b>, Scatter plots compared the Gtl2 and Rian gene expression between 4F/3F PαS-iPS and ES cells. <b>E</b>, Hierarchical clustering comparing the global gene expression between ES and iPS cells determined by DNA microarray. <b>F</b>, Scatter plots comparing the global gene expression between ES cells and source cells including PαS cells, OP cells, and TTF cells determined by DNA microarray.</p>