Germline chimeras and offspring from PαS-iPS cells.
A, Chimeric and F1 mice from 4F-PαS-iPS and 3F-PαS-iPS cells. 3F-PαS-iPS could observe nine over 50% chimerism chimeras. B, Germline-competent chimeric mice and F1 mice. Seven chimeras (>50% coloured coat colour) from 3F-PαS-iPS #1 and two from 3F-PαS-iPS #2 were bred with ICR females, and three of seven chimeas from 3F-PαS-iPS #1 and one of two chimera from 3F-PαS-iPS #2 produced iPS derived F1 mice. The ratio of pigmented F1 mice was observed. Numbers below each panel give the percentage of pigmented F1 mice. C, PCR analysis showing the presence of the GFP cassette in F1 mice obtained from the intercross between a chimeric male and an ICR female. D, Scatter plots compared the Gtl2 and Rian gene expression between 4F/3F PαS-iPS and ES cells. E, Hierarchical clustering comparing the global gene expression between ES and iPS cells determined by DNA microarray. F, Scatter plots comparing the global gene expression between ES cells and source cells including PαS cells, OP cells, and TTF cells determined by DNA microarray.