Figure_6.tif (378.65 kB)
Gentiolactone does not inhibit nuclear transport of NF-κB.
figure
posted on 2014-11-25, 02:44 authored by Hidetoshi Yamada, Sayaka Kikuchi, Tomoki Inui, Hideyuki Takahashi, Ken-ichi KimuraRAW264.7 cells were cultured for 1 hour with LPS (1 µg/mL) and gentiolactone (500 µM). The cytoplasmic or nuclear extracts were examined by immunoblotting analysis using anti-p65/NF-κB, anti-α-Tubulin or anti-Lamin B1 antibodies. Plotted values represent the mean value ± SD from four independent cultures. Helenalin was used as a positive control.