Generation and analysis of mice carrying a loss-of-function mutation in the <i>Tll2</i> gene.

2013-02-21T10:59:37Z (GMT) by Se-Jin Lee
<p>(a) Gene targeting strategy. Locations of exons 6–9 are shown as black boxes, and LoxP sites are denoted by triangles. (b) Northern analysis of <i>Tll2</i> expression levels. Twenty micrograms of poly A-selected brain RNA isolated from either wild type or <i>Tll2<sup>−/−</sup></i> mice were electrophoresed, blotted, and hybridized with a <i>Tll2</i> probe corresponding to exons 1–3. The blot was re-hybridized with a probe for the S26 ribosomal protein to control for loading. (c) Muscle weight increases in <i>Tll2<sup>−/−</sup></i> mice. Numbers represent percent increases relative to wild type mice and were calculated from the data shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001628#pone-0001628-t001" target="_blank">Table 1</a>. Muscles analyzed were: pectoralis (red), triceps (gray), quadriceps (blue), and gastrocnemius (green).</p>