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General scheme of the ‘mESC Plasticity Test’.
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posted on 2016-01-18, 14:26 authored by Aya Abou Hammoud, Nina Kirstein, Virginie Mournetas, Anais Darracq, Sabine Broc, Camille Blanchard, Dana Zeineddine, Mohamad Mortada, Helene Boeuf(A) mESCs maintained in pluripotency (+LIF) or cultivated without LIF for the indicated period of time in days (d1 to d5) are induced with LIF at different time points. (B) The effect of specific genes was tested by a siRNA strategy in which addition of siRNA was performed the first day of LIF withdrawal. (C) PI3K inhibitor LY was added for 24 hours to the committed or differentiated cells as indicated.
