Figure_6.tif (2.75 MB)
Gene expressions validated by RT-PCR and Western Blot analysis.
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posted on 2013-02-20, 08:07 authored by Yiwei Li, Dejuan Kong, Aamir Ahmad, Bin Bao, Fazlul H. SarkarBR-DIM and isoflavone regulated the expression of MITF, p38, Akt, NKX3-1, p27, cyclin D, CREB and PSA at mRNA and protein levels tested by real-time PCR (A, B) and Western Blot analysis (C, D). C4-2B (A, C) and PC-3 (B, D) cells were treated with 25 µM G2535 or 25 µM BR-DIM for 24 hours (for RNA extraction) or 48 hours (for protein extraction). (BD: BR-DIM).
