Gene expressions validated by RT-PCR and Western Blot analysis.

<p>BR-DIM and isoflavone regulated the expression of MITF, p38, Akt, NKX3-1, p27, cyclin D, CREB and PSA at mRNA and protein levels tested by real-time PCR (A, B) and Western Blot analysis (C, D). C4-2B (A, C) and PC-3 (B, D) cells were treated with 25 µM G2535 or 25 µM BR-DIM for 24 hours (for RNA extraction) or 48 hours (for protein extraction). (BD: BR-DIM).</p>