Gene expression of (A) long and (B) short form variants of <i>MS4A12</i> in human colon normal, adenomatous polyp and carcinoma tissue.

<p>Gene expression is normalised to reference gene <i>UBE2D2</i>. The asterisk (*) indicates significant decrease in expression levels of adenomatous polyp compared to normal, p<0.005. (C) – (D). <i>In situ</i> hybridisation of <i>MS4A12</i> transcripts in human colon (C) normal, (D) adenomatous polyp and (E) carcinoma. Emulsion autoradiographs showing expression of <i>MS4A12</i> at luminal epithelial surface (ep) of normal (C) in bright field and corresponding dark field images in antisense (left) and sense (right) hybridised tissue sections (n = 5). <i>MS4A12</i> is largely absent in adenomatous polyp (D) and localised in discrete areas of epithelium in carcinoma (E). Bar = 20 µm.</p>