Gating strategy for the characterization of cellular infiltrates in the spleen and for the sorting of leukocyte subpopulations.

<p>The leukocytes were gated based on the CD45 expression (P1) and only CD45+ cells were included in the sorting and analyses. Sorted populations were followed: CD4 T-lymphocytes (P4+P3), CD8+ T-lymphocytes (P5), γδ T-lymphocytes (P6), monocytes/macrophages (P7), B-lymphocytes (P8) and heterophils (P9).</p>