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Gating strategy for the characterization of cellular infiltrates in the spleen and for the sorting of leukocyte subpopulations.
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posted on 2013-02-20, 08:29 authored by Marta Matulova, Hana Stepanova, Frantisek Sisak, Hana Havlickova, Marcela Faldynova, Kamila Kyrova, Jiri Volf, Ivan RychlikThe leukocytes were gated based on the CD45 expression (P1) and only CD45+ cells were included in the sorting and analyses. Sorted populations were followed: CD4 T-lymphocytes (P4+P3), CD8+ T-lymphocytes (P5), γδ T-lymphocytes (P6), monocytes/macrophages (P7), B-lymphocytes (P8) and heterophils (P9).
