GM-CSF and IFN-γ levels of PBMCs from patients before surgery.

<p>In (<b>A</b>) GM-CSF ELISPOT of PBMCs was obtained from patients before surgery. Mononuclear cells were stimulated and were investigated for GM-CSF secretion. All experiments were carried out in triplicate. Results are presented as mean ± SD of the different groups. (<b>B</b>) IFN-γ ELISPOT of PBMCs was obtained from patients before surgery. Mononuclear cells were stimulated and were investigated for IFN-γ secretion. All experiments were carried out in triplicates. Results are presented as mean ± SD of the different groups. Large bar indicates comparison between patient stage D and stage D+Manumycin treatment samples.</p>

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