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GFP activity driven by linear reporter plasmid.

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posted on 2013-02-23, 11:26 authored by Leticia V. Bentancor, Marcos F. Bilen, María P. Mejías, Romina J. Fernández-Brando, Cecilia A. Panek, Maria V. Ramos, Gabriela C. Fernández, Martín Isturiz, Pablo D. Ghiringhelli, Marina S. Palermo

293 T cells were transfected with pr1-eGFP or pr7-eGFP linearized with Pcil restriction enzyme. After 48 h, cells were analyzed by fluorescence microscopy using Nikon Eclipse TE2000 microscope equipped with a CCD camera, using 1000X magnification. Green fluorescence photos were taken with 400 ms of exposure and 3.2 of gain. Numbers 1, 2, 3, 4 correspond to images visualized with white light, green filter, merge between DAPI and green filter and merge between white light and green filter, respectively. A. 293 T cells transfected with the Δpr-eGFP plasmid. B. Cells transfected with linear pr1-eGFP. C. Cells transfected with linear pr7-eGFP.

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