GFP activity driven by linear reporter plasmid.
2013-02-23T11:26:43Z (GMT) by
<p>293 T cells were transfected with pr1-eGFP or pr7-eGFP linearized with Pcil restriction enzyme. After 48 h, cells were analyzed by fluorescence microscopy using Nikon Eclipse TE2000 microscope equipped with a CCD camera, using 1000X magnification. Green fluorescence photos were taken with 400 ms of exposure and 3.2 of gain. Numbers 1, 2, 3, 4 correspond to images visualized with white light, green filter, merge between DAPI and green filter and merge between white light and green filter, respectively. <b>A.</b> 293 T cells transfected with the Δpr-eGFP plasmid. <b>B.</b> Cells transfected with linear pr1-eGFP. <b>C.</b> Cells transfected with linear pr7-eGFP.</p>