Functional analysis of HAE in vitro auto-phosphorylation sites.

A) Identified HAE auto-phosphorylation sites annotated on the amino acid sequence of the HAE intracellular domain. Conserved features of protein kinases, as well as the HAE specific juxtamembrane region and C-terminal tail, are annotated for reference. B) Ratio of mutant:wildtype in situ auto-phosphorylation levels of recombinant MBP-HAE mutants. N = 4 biological replicates. Wildtype is normalized to 1. Error bars represent the standard deviation. Tests for statistical significance were carried out by a one sample t-test. C) Representative stains for phosphorylation and total protein by Pro-Q Diamond and coomassie blue, respectively.