Functional Display of IA<sup>b</sup>-p3K on the Surface of Insect Cells

<div><p>(A) Sf9 insect cells were infected with baculovirus encoding a membrane-bound form of IA<sup>b</sup>-p3K. After 3 d, the surface expression of IA<sup>b</sup>-p3K was detected with an anti-IA<sup>b</sup> mAb using flow cytometry.</p> <p>(B) The genes for mouse ICAM (CD54) and B7.1 (CD80) were cloned into an insect cell expression plasmid as described in the <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0020090#s4" target="_blank">Materials and Methods</a>. The plasmids were used to cotransfect Sf9 cells, and a stable transfectant (Sf9-ICAM/B7.1) was cloned expressing both proteins detected with mAbs using flow cytometry.</p> <p>(C) Either Sf9 (open bars) or Sf9-ICAM/B7.1 (closed bars) cells were infected with baculovirus expressing IA<sup>b</sup>-p3K. After 3 d, the infected insect cells were used as APCs to stimulate IL-2 production from B3K-06 and YAe-62. Uninfected cells were used as negative controls.</p></div>